Catalog

Review our options for custom primers, probes, oligonucleotides and other common products for PCR, qPCR (real-time PCR), functional genomics, genotyping, gene expression, and more, with easy-to-use ordering tools, fast and convenient delivery, and operative customer support options.

Personalize probes according to your requirements and application. Below you will find oligonucleotide variants with fluorescent dyes and/or quenchers whose design criteria provide maximum flexibility for assays while maintaining high quality and reproducibility of results.

View information on the chemical modifications of oligonucleotides available for order. If your desired modification is not listed in this section, please contact us. We will endeavor to review and respond to your request as soon as possible.

Note! Most of the modifications are introduced during the solid-phase synthesis of oligonucleotides. Please note that some modifications are not stable under synthesis conditions, so they are introduced after synthesis, which leads to a decrease in product yield due to additional procedures and purification steps.

Reverse Transcriptases (also known as revertases) are enzymes that are used to generate complementary DNA (cDNA) at RNA templates. This step is the must before PCR or LAMP, when one works with RNA targets. Choose a revertase for your assay from our selection list below.

As PCR can amplify such tiny amounts of DNA, preventing contamination is essential: even small amounts of contamination can produce false positives in your experiments. Contamination can include cross-contamination from other samples, DNA contamination from elsewhere in the laboratory, and carryover contamination from amplification products and primers used in prior PCR experiments. During PCR, deoxyuridine triphosphate (dUTP) can be substituted for deoxythymidine triphosphate (dTTP) in the synthesis of product DNA. Thus to reduce the frequency of false positive results due to amplicon contamination, one common recommendation has been to substitute dUTP for dTTP as a source of nucleotides for the PCR reaction. Amplicon DNA that has incorporated dUTP can then be degraded with uracil-DNA glycosylase prior to subsequent amplification reactions, thus preventing these molecules from producing false positive results by acting as template.

Magnetic beads technology is one of the emerging strategies for extracting RNA and genomic, plasmid, and mitochondrial DNA. The technique involves the separation of nucleic acids from complex mixtures like blood, tissues, and others and is relatively easy to execute, being one of the best choices for automation, high-throughput applications, and high sample processivity. very rate