- Life Science
- In vitro diagnostics
- Synthesis of oligonucleotides
- Primers
- Fluorescently labeled probes and samples
- Oligonucleotides GentaOligoPure
- Modifications
- ‘Click’ chemistry modifications
- 2'-F (2'-Fluoro) RNA modification
- Phosphorothioate bonds
- 2'-MOE (2'-O-Methoxyethyl) RNA modification
- 2'-OMe (2'-O-Methyl) RNA modification
- LNA (Locked Nucleic Acid) RNA modification
- Other modifications
- Phosphorylation
- Thiophosphate bonds
- Fluorophores and quenchers
- Linkers and functionalization
- Reagents for molecular biology
- Services
Phosphorylation
3’—Phosphorylation. Chemical 3’-phosphorylation is used to block oligonucleotide elongation by DNA polymerases lacking 3’→5’ exonuclease activity.
5’-Phosphorylation. Chemical 5’-phosphorylation is an efficient alternative to enzymatic procedures that simplify a working layout and save time. 5’-Phophorylated oligonucleotides are used in gene assembly, ligase chain reaction and construction of DNA nanostructures [DOI: 10.1039/C8CC00738A].
Available sites for modifications are 5 and 3’.>
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