siRNA
RNA interference (RNAi) using chemically synthesized small interfering RNA (siRNA) is another straightforward way to perform transient knockdown of gene expression to study functions of proteins and non-coding RNA in cell culture, primary cells and in animals. Each siRNA is unwound into two single-stranded RNAs: the sense strand and the guide -antisense strand. The sense strand is then cleaved by the protein Argonaute 2 and degraded and the guide strand is incorporated into the RNA-induced silencing complex RISC. The RISC assembly then binds and degrades the target mRNA by inducing the cleavage by Ago2, a catalytic component of the RISC [C. Matranga et al, 2005].
We offer inhouse design of siRNAs for many species, chemical synthesis and validation in vitro. To validate active siRNA we can use cell lines (list) or use luciferase reporters if your gene of interest is not highly expressed in available cell lines. We always introduce chemical modifications, such as 2′-O-methyl (2’-OMe), 2’-fluoro (2’-F) and phosphorothioates to increase nuclease stability and decrease immune response. If you require assistance for planning your project, please contact us [email protected].
- Highly specific siRNA - our in-house design reduces possible off-target effects
- Chemically modified siRNA with improved stability
- Dedicated purification and thorough quality control
You can fill out the feedback form to discuss the project and/or get more detailed information about this service.