Catalog

Gene knockout using CRISPR technology is a recognized tool in biology and biotechnology. The most common technique relies on guide RNA (gRNA) Cas9-mediated dsDNA breaking is followed by reparation of DNA by multiple mechanisms. gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20 nucleotide spacer that defines the genomic target to be modified. Several DNA reparation processes in the cell often lead to the generation of errors, indels and frameshifts in DNA that disrupt the protein-coding capacity of the locus. The main issue of Cas9 technology are off-target effects, so robust selection of the knockout clones is crucial for success.

We offer in-house design of gRNA that target genes of your interest, cloning of gRNA in the vector for in vitro studies and generation of KO cell lines.

If you need help with project planning, please contact us by email: info@genterra.ru or via the feedback form.