- Life Science
- In vitro diagnostics
- Synthesis of oligonucleotides
- Primers
- Fluorescently labeled probes and samples
- Oligonucleotides GentaOligoPure
- Modifications
- ‘Click’ chemistry modifications
- 2'-F (2'-Fluoro) RNA modification
- Phosphorothioate bonds
- 2'-MOE (2'-O-Methoxyethyl) RNA modification
- 2'-OMe (2'-O-Methyl) RNA modification
- LNA (Locked Nucleic Acid) RNA modification
- Other modifications
- Phosphorylation
- Thiophosphate bonds
- Fluorophores and quenchers
- Linkers and functionalization
- Reagents for molecular biology
- Services
2’-deoxy-Uridine (dU)
2’-deoxyuridine (dU) can be used instead of thymidine residues in DNA oligonucleotides. The most frequent mutation in the cell – deamination of dC in DNA results in dU formation. dU in DNA is recognized by DNA reparation enzymes - uracil-DNA-glycosylases which subsequently removes uracil followed by strand scission and reparation.
This system can be used to decrease lab contamination with PCR products. As dUTP is a good substrate for Taq DNA polymerase, so one can fully or partially substitute dTTP with dUTP. Addition of thermolabile uracil-DNA-glycosylase to the test tube allows eliminate DNA contamination, while heating during PCR lead to UDG denaturation without any interference with further amplification.
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