2'-OMe (2'-O-Methyl) RNA modification

2’-OMe modification in oligonucleotides provide several features like rapid hybridization kinetics to complementary RNA, thermal stability of duplexes, and resistance to nucleases. This modification is beneficial for Antisense oligonucleotides and siRNA. However, duplexes of fully 2′-OMe-modified oligonucleotides with RNA does not support RNase H activity, so gapmer approach is needed to resolve this issue.

Available sites for modifications are 5’, internal, and 3’.