Catalog

The creation of reporter constructs with luciferase is a method that allows to study the transcription and regulation of genes by measuring the luminescence of luciferase, which is expressed under a given promoter. Cells are transfected by a genetic construct(s), one of which contains the reporter gene under study, which is under the control of a certain promoter, and the second vector carries a control reporter gene under the control of a constitutive promoter. Analysis with double luciferase is carried out through sequential measurement of the activity of firefly luciferase and Renilla luciferase in the same sample on a luminometer. The result of this analysis is the relationship between the activities of firefly luciferase (Fluc) and Renilla luciferase (Rluc).

JSC "GenTerra" provides services for planning and conducting work with cell cultures and measuring the activity of luciferase.