Catalog

Locked nucleic acids have restricted conformation of the sugar moiety formed by methylene bridge between oxygen atom at 2’-position and carbon at 4’-position. Such predefined conformation of ribose determines excellent hybridization of oligonucleotides even if they bear only 3-5 LNA residues. LNAs can be inserted into both RNA and DNA oligonucleotides, and their presence increases the Tm by 2 to 4° per LNA. LNAs are also widely applied because they enhance hybridization stability and biostability of oligonucleotides in vivo [https://doi.org/10.1016/B978-0-12-407676-1.00002-0]. LNA oligonucleotides are used in ASO, primers and probes for PCR, in ribozymes, FISH probes (fluorescent in situ hybridization), molecular beacons for SNP (single nucleotide polymorphism) identification, microarrays, etc.

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