Genta REV M Revertase
Genta RevM is a recombinant reverse transcriptase with increased thermostability compared to ‘wild type’ enzyme, therefore, it has optimal working temperature range 45-55 °C, and it maintains its activity up to 60 °C. It is a perfect choice for “one step” RT-PCR, as well as for synthesis of full-length cDNAs (up to 10 kb) for work with GC-rich long mRNA templates.
Features and Advantages
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High efficacy
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Increased thermolability
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Optimal reaction temperature 50°C
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Suitable for difficult RNA templates
Genta REV M Revertase,
100,000 units/ml - 0.1 ml
Buffer Genta-B 2 - 1.25 ml
Genta-T4 DNA ligase,
400,000 units/ml - 1 x 0.05 ml
10x buffer - 1 x 0.5 ml
Genta REV M Revertase,
100,000 units/ml - 5 x 0.1 ml
Buffer Genta-B 2 - 5 x 1.25 ml
Genta REV M Revertase,
100,000 U/ml - XXX ml
no buffer
* minimum order quantity - 2 ml
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(under the order)*
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10 000 units
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20 000 units
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50 000 units
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- Артикул
- RP-E-005-Custom
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(under the order)*
-
10 000 units
-
20 000 units
-
50 000 units
-
-
- Артикул
- RP-E-005-Custom
- Product information
Concentration: 100,000 U/ml
Scope of application:
- cDNA synthesis
- Single-step RT-PCR
- Preparation of DNA libraries
- Insert labeled nucleotides into cDNA
Main properties:
- The optimum temperature for enzyme activity is 50°C
- High yield cDNA
- Synthesis of long cDNA
- Improved efficiency with GC-rich RNA templates
- Other characteristics
Storage buffer: 20 mM Tris-HCl (pH 7.5 at 25°C); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.01% Igepal CA-630; 50% glycerin.
Storage temperature: - 20°C.
Transportation conditions: in a thermal container with ice packs, at a temperature of 2°C to 8°C for no more than 7 days.
Shelf life under the conditions of storage and transportation: 24 months from the date of production.