Catalog

Hybridization probes (FRET probes)

FRET probes are hybridization probes based on the Förster resonance energy transfer (FRET). FRET probes are a pair of fluorescent probes placed in close proximity on the targer. Fluorophores are so chosen that the emission spectrum of one overlaps significantly with the excitation spectrum of the other. During FRET, the donor fluorophore excited by a light source, transfers its energy to an acceptor fluorophore when positioned in the direct vicinity of the former. The acceptor fluorophore emits light of a longer wavelength, which is detected in specific channels. The light source cannot excite the acceptor dye. Usually it’s a pair of oligonucleotides where one has 3’-Donor Dye and another with 5’ -Acceptor dye.

Applications: RT-qPCR, DNA copy number measurement, pathogen detection assays, SNP genotyping.
Multiplexing
Choose from a wide variety of reporter and quencher combinations

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Modifications

Recommended FRET Pair dyes combinations

Acceptor Dye	Donor Dye
ROX	FAM
Cy5	FAM

The set of hybridization FRET probes consist of two oligonucleotides labeled with donor and acceptor dyes (a FRET pair) that can come in close proximity only after binding to the target. Without target DNA FRET between probes is absent and direct excitation of the acceptor dye is impossible, while upon binding to the target one can observe energy transfer as dyes are located in close defined proximity. If a donor fluorophore is excited by an external light source, a part of excitation energy is transferred to the adjacent acceptor fluorophore followed by emission.