Genta TaqF DNA polymerase
Genta TaqF DNA polymerase is a chemically modified recombinant analogue of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5'-3' polymerase activity and 5'-3' exonuclease activity, but no 3'-5' corrective exonuclease activity. The recombinant Genta TaqF DNA polymerase enzyme is suitable for use in routine PCR, including real-time PCR.
Genta TaqF DNA polymerase is inactivated by chemical modification, which allows the preparation of the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of the amplification. Polymerase activation occurs when it is heated to 95°C for 15 minutes.
Genta TaqF DNA Polymerase,
5,000 units/ml - 0.2 ml
5x Genta PCR buffer - 2 x 1.25 ml
Genta TaqF DNA Polymerase,
5,000 units/ml – XXX ml
no buffer
*minimum order quantity - 5 ml
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(under the order)*
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1 000 units
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1х1.25 ml
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- Артикул
- RP-E-003-Custom
-
(under the order)*
-
1 000 units
-
1х1.25 ml
-
-
- Артикул
- RP-E-003-Custom
- Product information
- Concentration: 5,000 units/ml
Scope of application:
- Routine PCR of DNA fragments up to 5 kb
- Multiplex PCR
- Low copy PCR
- Real-time PCR
- Insert labeled oligonucleotides
- Hot start
- Improved amplification specificity compared to Taq DNA polymerase
- The optimum temperature for enzyme activity is 72-74°C
- 5'-3' exonuclease activity
- The length of the amplified fragments is up to 5000 bp
- Ability to clone PCR products into TA-vector
- Ability to use uracil-containing primers and templates for PCR
- Routine PCR of DNA fragments up to 5 kb
- Other characteristics
Storage buffer: 20 mM Tris/HCl, pH 8.3 (at 25°C); 100 mM KCl; 0.1 mM EDTA; 50% glycerin; stabilizers.
Reaction buffer (1x PCR buffer): 70 mM Tris/HCl (pH 8.8 at 25°C); 17 mM (NH4)2SO4; 4 mM MgSO4; 0.01% tween-20; 0.1 mg/ml BSA; 8% glycerin.
At the request of the customer, Genta TaqF DNA polymerase can be completed with a buffer without Mg2+ , with 10 mM MgSO4 (5x PCR buffer) or according to customer's prescription.
Storage temperature: - 20°C.
Transportation conditions: in a thermal container with ice packs, at a temperature of 2°C to 8°C for no more than 7 days.
Shelf life under the conditions of storage and transportation: 24 months from the date of production.