Catalog

Genta-ExoI exonuclease I

Genta-ExoI exonuclease I hydrolyzes single-stranded DNA in the 3' → 5' direction, releasing deoxyribonucleoside 5'-monophosphate. Does not cleave DNA strands, the 3'-end of which is blocked by a phosphoryl or acetyl group. Active in PCR buffer.

Isolated from a strain of Escherichia coli expressing the E. coli exonuclease I gene.

Product

Description

Cat. №

Packing

Quantity

Price, RUB

Genta-ExoI exonuclease I

Equipment:
Genta-ExoI exonuclease I,
20,000 units/ml - 1 x 0.2 ml
10x Genta ExoI buffer - 1 x 0.5 ml

RP-E-017-S

4 000 units

Price on request

Genta REV L Revertase

Equipment:
Genta REV L Revertase,
300,000 units/ml - 5 x 0.1 ml
Buffer Genta-B 2 - 5 x 1.25 ml

RP-E-010-L

150 000 units

Price on request

Genta-ExoI exonuclease I

Equipment:
Genta-ExoI exonuclease I,
20,000 U/ml - 5 x 0.2 ml
10x Genta ExoI buffer - 2 x 1.25 ml

RP-E-017-L

20 000 units

Price on request

Concentration: 20,000 units/ml

Scope of application:
- Digestion of primers in PCR product prior to sequencing or SNP analysis
- Primer hydrolysis before nested PCR
- Cleavage of linear single-stranded DNA in double-stranded DNA samples
Main Features:
- Reaction conditions: 15-30 minutes at 37°C, 10 units. Genta-ExoI exonuclease I
- Exonuclease I inactivation: 15 minutes at 80°C.
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Storage buffer: 20 mM Tris-HCl (pH 8.3 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM 1-thioglycerol; 0.5% Twin-20; 50% glycerin.

10xExoI buffer: 670 mM glycine/KOH (pH 9.5 at 25°C); 67 mM MgCl2; 100 mM mercaptoethanol.
Thermal inactivation: 15 minutes at 80°C.

Storage temperature: from minus 24°C to minus 16°C

Transportation conditions: in a thermal container with ice packs, at a temperature of 2°C to 8°C, no more than 5 days.

Shelf life under the conditions of storage and transportation: 24 months from the date of production.

Объём

150 000 units
20 000 units
4 000 units
-

Артикул

RP-E-017-Custom

Product information

Concentration: 20,000 units/ml

Scope of application:

Digestion of primers in PCR product prior to sequencing or SNP analysis
Primer hydrolysis before nested PCR
Cleavage of linear single-stranded DNA in double-stranded DNA samples

Main Features:

Reaction conditions: 15-30 minutes at 37°C, 10 units. Genta-ExoI exonuclease I
Exonuclease I inactivation: 15 minutes at 80°C.

Other characteristics

Storage buffer: 20 mM Tris-HCl (pH 8.3 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM 1-thioglycerol; 0.5% Twin-20; 50% glycerin.

10xExoI buffer: 670 mM glycine/KOH (pH 9.5 at 25°C); 67 mM MgCl2; 100 mM mercaptoethanol.
Thermal inactivation: 15 minutes at 80°C.

Storage temperature: from minus 24°C to minus 16°C

Transportation conditions: in a thermal container with ice packs, at a temperature of 2°C to 8°C, no more than 5 days.

Shelf life under the conditions of storage and transportation: 24 months from the date of production.

On request

pcs.

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