Genta benzonuclease, ≥99%
Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and circular) to form oligodeoxynucleotides with terminal 5'-monophosphates 3-5 bases long. Effective over a wide range of operating conditions. Has no proteolytic activity.
Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens.
Reaction conditions: 20 - 37°C, optimal buffer: 50 mm Tris-HCl (pH 8.0 at 25°C); 100-150 mM NaCl; 1 mM MgCl2.
Product
Description
Cat. №
Packing
Quantity
Price, RUB
10x Genta TE buffer
RP-B-013-L
100 ml
Price on request
10x Genta TE buffer
RP-B-013-X
(under the order)*
Price on request
Genta benzonuclease, ≥99%
Equipment:
Genta benzonuclease, ≥99%,
250,000 units/ml - 1 x 0.2 ml
Genta benzonuclease, ≥99%,
250,000 units/ml - 1 x 0.2 ml
RP-E-016-S
50 000 units
Price on request
Genta benzonuclease, ≥99%
Equipment:
Genta benzonuclease, ≥99%,
250,000 units/ml - 5 x 0.2 ml
Genta benzonuclease, ≥99%,
250,000 units/ml - 5 x 0.2 ml
RP-E-016-L
250 000 units
Price on request
Genta benzonuclease, ≥99%
Equipment:
Genta benzonuclease, ≥99%,
250,000 units/ml - XXX ml
* minimum order quantity - 5 ml
Genta benzonuclease, ≥99%,
250,000 units/ml - XXX ml
* minimum order quantity - 5 ml
RP-E-016-X
(under the order)*
Price on request
-
(under the order)*
-
100 ml
-
250 000 units
-
50 000 units
-
-
- Артикул
- RP-E-016-Custom
Объём
-
(under the order)*
-
100 ml
-
250 000 units
-
50 000 units
-
-
- Артикул
- RP-E-016-Custom
- Product information
- Concentration: 250 000 units/ml
Scope of application:
- Purification of viral vaccines, viral vectors for vaccines
- DNA/RNA removal from protein preparations and other biological products
- Reducing the viscosity of solutions caused by the presence of NK
- Prevent cell aggregation
- NA cleavage in inclusion bodies before renaturation of insoluble proteins
- Sample preparation for electrophoresis and chromatography
- The specific activity of the enzyme preparation is at least 1.5 x 106 act. units/mg of protein.
- A unit of benzonuclease activity is taken as the amount of an enzyme that converts 50 µg of sonified salmon sperm DNA into nucleotides in 30 minutes at 37°C in reaction buffer: 50 mM Tris/HCl, pH 8.3, 5 mM MgCl2, 1 mM DDT.
- Purity: ≥ 99% (SDS-PAGE)
- Other characteristics
- Storage buffer: 50 mM Tris/HCl, pH 8.0 (at 25°C); 50 mM NaCl; 2 mM MgCl2; 50% glycerin.
Storage temperature: from minus 24°С to minus 16°С.
Transportation conditions: in a thermal container with ice packs, at a temperature of 2°C to 8°C, no more than 7 days.
Shelf life under the conditions of storage and transportation: 24 months from the date of production.
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